H-Antigen in HeLa Cells and H-Antibodies in Anti-HeLa Sera

Anti-idiotypes to monoclonal anti-H-2 antibodies. II. Expression of anti-H-2Kk idiotypes on antibodies induced by anti-idiotype or H-2Kk antigen

Anti-idiotypic antibodies were prepared against two monoclonal anti-H-2Kk antibodies, 11-4.1 and 3-83P. These reagents were used to examine idiotype (Id) expression on anti-H-2Kk antibodies induced by the in vivo administration of the anti-idiotypic antibodies and/or H-2Kk antigen. Treatment of BALB/c mice with anti-Id induced both antigen-binding and nonantigen-binding Id-positive molecules in...

Subcompartments in Intact HeLa Cells

Two recombinant aequorin isoforms with different Ca 2 1 affinities, specifically targeted to the endoplasmic reticulum (ER), were used in parallel to investigate free Ca 2 1 homeostasis in the lumen of this organelle. Here we show that, although identically and homogeneously distributed in the ER system, as revealed by both immunocytochemical and functional evidence, the two aequorins measured ...

Rna Synthesis in Hela Cells

Interphase HeLa cells manifest a stepwise shutoff of RNA synthesis when the tonicity of the extracellular medium is gradually increased. Synthesis of heterogeneous nuclear RNA is most sensitive and is selectively inhibited at 1.5 times isotonicity (450 milliosmols/liter), while 45S ribosomal RNA synthesis is not affected significantly below 2.0 times isotonicity. Transfer RNA synthesis is least...

Comparative titration of Poliomyelitis antibodies in Monkey Kidney, Hela, and FL Amnion cell

Electrolyte Metabolism in HeLa Cells

Methods have been developed to study cellular Na, K, and Cl concentrations in HeLa cells. Cell [Na] and [K] are functions of the age of the culture. As the culture grows [K], expressed in mmols/liter cell H(2)O, rises from an initial value of 121 to a peak of 206 at about 4 days, and thereafter falls until it has almost returned to the initial value by the 9th day. [Na] falls as [K] rises, but ...